cd16 monocytes (Miltenyi Biotec)

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Miltenyi Biotec cd16 monocytes
Cd16 Monocytes, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 215 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd16+monocytes/CD16+MicroBeads%2C+human/pm41689998-74-20-30
Average 95 stars, based on 215 article reviews

cd16 monocytes - by Bioz Stars, 2026-09

95/100 stars

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Magnetic Cell Separation:

Article Title: Platelet extracellular vesicles drive monocyte differentiation into inflammatory foam cells in peripheral arterial disease.
Article Snippet: .. Collected cells were resuspended in 400 ml MACS buffer and incubated with anti-CD16 human microbeads at 4◦C for 20 min. CD16+ monocytes were then positively selected using as LS column (Miltenyi; see above) and eluted using 5 ml MACS buffer, followed by cell counting. .. Monocytes were resuspended in pre-warmed 0.15 % (v/v) BSA (Gibco) in RPMI (Gibco) at a concentration of 7.0 × 105/ml for culture in a 24 well tissue culture plate (Falcon) or 7.5 × 105/ml for culture in 8 well μ-slide chambers (Ibidi).

Incubation:

Cell Counting:

Isolation:

Article Title: Molecular Characterization of Monocyte Subsets Reveals Specific and Distinctive Molecular Signatures Associated With Cardiovascular Disease in Rheumatoid Arthritis
Article Snippet: .. Firstly, CD16 + monocytes were isolated through positive selection (using human anti-CD16, CD16 + monocyte isolation kit, MiltenyiBiotec, Bergisch Gladbach, Germany). .. Thereafter, the CD14 + monocytes were isolated from the eluted fluid using positive selection (human anti-CD14, CD14 microbeads, MiltenyiBiotec).

Article Title: Molecular Characterization of Monocyte Subsets Reveals Specific and Distinctive Molecular Signatures Associated With Cardiovascular Disease in Rheumatoid Arthritis.
Article Snippet: .. Firstly, CD16+ monocytes were isolated through positive selection (using human antiCD16, CD16+ monocyte isolation kit, MiltenyiBiotec, Bergisch Gladbach, Germany). .. Thereafter, the CD14+ monocytes were isolated from the eluted fluid using positive selection (human anti-CD14, CD14 microbeads, MiltenyiBiotec).

Article Title: Conjugates for targeted cell surface editing
Article Snippet: Monocytes were isolated from PBMCs using the Pan Monocyte isolation kit (Miltenyi Biotec 130-096-537). .. CD16+ monocytes were isolated from PBMCs using the CD16+ Monocyte isolation kit (Miltenyi Biotec 130-091-765). .. After isolating fresh PBMCs, M1 and M2-polarized macrophages were acquired by first plating freshly isolated PBMCs in serum-free RPMI a T75 flask (Fisher Scientific 1368065) at 37° C. in 5% CO2 for 2 hours, then removing media and washing cells three times with phosphate buffered saline (PBS +Ca +Mg) to isolate monocytes.

Article Title: Methods of treating conditions with antibodies that bind colony stimulating factor 1 receptor (CSF1R)
Article Snippet: .. Nonlimiting exemplary methods of determining the levels of CD16+ and/or CD16− monocytes include methods provided by commercial kits, such as CD16+ Moncyte Isolation Kit (Miltenyl Biotec, Bergisch Gladbach, Germany). ..

Selection:

Article Title: Molecular Characterization of Monocyte Subsets Reveals Specific and Distinctive Molecular Signatures Associated With Cardiovascular Disease in Rheumatoid Arthritis.
Article Snippet: .. Firstly, CD16+ monocytes were isolated through positive selection (using human antiCD16, CD16+ monocyte isolation kit, MiltenyiBiotec, Bergisch Gladbach, Germany). .. Thereafter, the CD14+ monocytes were isolated from the eluted fluid using positive selection (human anti-CD14, CD14 microbeads, MiltenyiBiotec).

other:

Article Title: HIV-infected cannabis users have lower circulating CD16 + monocytes and IP-10 levels compared to non-using HIV patients
Article Snippet: PBMCs were isolated from human leukocyte packs (Gulf Coast Regional Blood Center, Houston, TX) of HIV-MJ− donors and whole blood of HIV+ (MJ− and MJ+) donors by density gradient centrifugation using Ficoll-Paque PLUS (GE Healthcare Life Sciences, Pittsburgh, PA).

Article Title: HIV-infected cannabis users have lower circulating CD16 + monocytes and IP-10 levels compared to non-using HIV patients
Article Snippet: Peripheral blood mononuclear cell (PBMC) and CD16 − monocyte isolation for in vitro studies PBMCs were isolated from human leukocyte packs (Gulf Coast Regional Blood Center, Houston, TX) of HIV-MJ− donors and whole blood of HIV+ (MJ− and MJ+) donors by density gradient centrifugation using Ficoll-Paque PLUS (GE Healthcare Life Sciences, Pittsburgh, PA).

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